Search results for "RT-PCR t"

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Cardiac surgery outcomes in patients with coronavirus disease 2019 (COVID-19): A case-series report

2020

Abstract Objective The impact of coronavirus disease 2019 (COVID-19) on the postoperative course of patients after cardiac surgery is unknown. We experienced a major severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak in our cardiac surgery unit, with several patients who tested positive early after surgery. Here we describe the characteristics, postoperative course, and laboratory findings of these patients, along with the fate of the health care workers. We also discuss how we reorganize and reallocate hospital resources to resume the surgical activity without further positive patients. Methods After diagnosis of the first symptomatic patient, surgery was suspended. Naso…

AdultMalePulmonary and Respiratory MedicineSIRS systemic inflammatory response syndromemedicine.medical_specialtyInfectious Disease Transmission Patient-to-ProfessionalCoronavirus disease 2019 (COVID-19)Sars-CoV-2medicine.medical_treatmentSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)cardiac surgery COVID-19 new coronavirus SARS-CoV-2CRP C-reaction protein030204 cardiovascular system & hematologyHealth Services AccessibilityArticleDisease OutbreaksTertiary Care Centers03 medical and health sciences0302 clinical medicinePostoperative ComplicationsValve replacementInterquartile rangeInternal medicineHealth caremedicineHumansIn patientCardiac Surgical ProceduresAgedAged 80 and overInfection ControlHealth Care RationingTAVR transcatheter aortic valve replacementbusiness.industrynew coronavirusExtracorporeal circulationCOVID-19Middle AgedCardiac surgery030228 respiratory systemItalyRT-PCR t real-time reverse transcription polymerase chain reactionFemaleSurgeryCOVID-19 / Sars-CoV-2 novel coronavirus 2businessCardiology and Cardiovascular Medicinecardiac surgeryFollow-Up Studies
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Simultaneous detection of the seven main tomato-infecting RNA viruses by two multiplex reverse transcription polymerase chain reactions

2012

Cucumber mosaic virus, Tomato spotted wilt virus, Tomato mosaic virus, Tomato chlorosis virus, Pepino mosaic virus, Torrado tomato virus and Tomato infectious chlorosis virus cause serious damage and significant economic losses in tomato crops worldwide. The early detection of these pathogens is essential for preventing the viruses from spreading and improving their control. In this study, a procedure based on two multiplex RT-PCRs was developed for the sensitive and reliable detection of these seven viruses. Serial dilutions of positive controls were analysed by this methodology, and the results were compared with those obtained by ELISA and singleplex versions of RT-PCR. The multiplex and…

Serial dilutionSensitivity and SpecificityVirusPlant VirusesCucumber mosaic virusVirologyDiagnosisRNA VirusesMultiplexTomato mosaic virusSicilyVirus classificationPolymeraseMultiplex RT-PCRPlant DiseasesbiologyReverse Transcriptase Polymerase Chain ReactionfungiSettore AGR/12 - Patologia Vegetalefood and beveragesMICROBIOLOGIAMultiplex RT-PCR Tomato viruses Detection DiagnosisTomato virusesbiology.organism_classificationVirologyReverse transcriptaseDetectionItalybiology.proteinMultiplex Polymerase Chain Reaction
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